Bone Regeneration of Decellularized In-Vivo Deposited Extracellular Matrix (ECM) on Hydroxyapatite Sponge Scaffold

Submit Manuscript | http://medcraveonline.com of polysaccharides and proteins, such as collagens, proteoglycans and glycoproteins that are essential in tissue engineering [3]. Cellular interactions with the ECM are known to play a critical role in providing structural support to cells, directing cell function, and regulating development, homeostasis, and repair of a variety of tissues [4-8]. ECM derived and ECM mimicking scaffolds have shown promising results in facilitating the constructive remodeling of many different tissues in preclinical and applications [3,9,10]. To obtain an ECM derived or ECM mimicking scaffolds, decellularization process were employed in native tissues and organs to remove the cellular and nuclear contents while maintaining the important proteins present in the ECM. Studies have been focus on the generation of cell derived ECM for different tissue engineering applications [11-13]. However, different factors need to be considered in generation of cell derived ECM such as type of cells and the manner in which cell are cultured that involves the use of appropriate media, addition of growth factors, bioreactor design and flow rate [14]. The aim of this study is to determine the feasibility and effect of in-vivo deposited extracellular matrix on bone regeneration capacity of porous hydroxyapatite sponge scaffold.